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Calcium sulfate acts on the miRNA of MG63E osteoblast-like cells

โœ Scribed by Annalisa Palmieri; Furio Pezzetti; Giorgio Brunelli; Luca Scapoli; Lorenzo Lo Muzio; Antonio Scarano; Marcella Martinelli; Francesco Carinci

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
201 KB
Volume
84B
Category
Article
ISSN
1552-4973

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โœฆ Synopsis

Abstract

Calcium sulfate (CaS) is a highly biocompatible material and enhances bone formation in vivo. However, how CaS alters osteoblast activity to promote bone formation is incompletely understood. We therefore investigated the translation regulation in osteoblasts exposed to CaS by using microRNA microarray techniques. Transduction, transcription, and translation are the three levels of regulation of cell activity. Recently, a new type of translation regulation has been identified: RNA interference (RNAi). RNAi is a process in which microRNA, (miRNA), that is, noncoding RNAs of 19โ€“23 nucleotides can induce sequenceโ€specific mRNA degradation and/or translational repression. The human genome encodes a few hundred miRNAs that can postโ€transcriptionally repress thousands of genes. The miRNA oligonucleotide microarray provides a novel method of carrying out genomeโ€wide miRNA profiling in human samples. By using miRNA microarrays containing 329 probes designed from Human miRNA sequences, we identified in osteoblastโ€like cells line (MGโ€63) cultured with CaS (Surgiplaster, Classimplant, Roma, Italy) several miRNA whose expression is significantly modified. The data reported are, to our knowledge, the first study on translation regulation in osteoblasts exposed to CaS. They could be relevant to a better understanding of the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects. ยฉ 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2008

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