Calcium Signaling Protocols

✍ Scribed by Alec W. M. Simpson (auth.), David G. Lambert (eds.)

• Publisher: Humana Press
• Year: 2005
• Tongue: English
• Leaves: 372
• Series: Methods in Molecular Biology 312
• Edition: 2
• Category: Library

✦ Synopsis

Now extensively updated and expanded, Calcium Signaling Protocols, Second Edition continues the excellence of the highly regarded first edition. New to this edition are chapters on the electrophysiological measurement of Ca2+ channel activity, enhanced coverage of confocal microscopy, and practical tutorials on two of the most common industrial high-throughput machines, the Fluorometric Imaging Plate Reader (FLIPR), and the FlexStation. All experimental chapters provide readily reproducible techniques for measuring the many facets of Ca2+ signaling. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Calcium Signaling Protocols, Second Edition provides for novice and expert researchers alike both theoretical and practical information for the optimal design and performance of studies of intracellular Ca2+ regulation.

✦ Table of Contents

Front Matter....Pages i-x
Front Matter....Pages 1-1
Front Matter....Pages 3-36
Front Matter....Pages 37-45
Front Matter....Pages 47-53
Front Matter....Pages 55-55
Front Matter....Pages 57-85
Front Matter....Pages 87-117
Back Matter....Pages 119-124
....Pages 125-131

✦ Subjects

Biochemistry, general