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Calcium phosphate transfection optimization for serum-free suspension culture

โœ Scribed by Philippe Girard; Luc Porte; Temugin Berta; Martin Jordan; Florian M. Wurm

Book ID
110318910
Publisher
Springer
Year
2001
Tongue
English
Weight
364 KB
Volume
35
Category
Article
ISSN
0920-9069

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Optimization of Calcium Phosphate Transf
Optimization of Calcium Phosphate Transfection for Bovine Chromaffin Cells: Relationship to Calcium Phosphate Precipitate Formation
โœ S.P. Wilson; F. Liu; R.E. Wilson; P.R. Housley ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 934 KB

Optimal conditions for formation of calcium phosphate-DNA precipitates and for chromaffin cell transfection by the calcium phosphate method were examined. A relationship was observed between turbidity of calcium phosphate solutions and the ability of calcium phosphate-DNA mixtures to give efficient