Calcium measurements in organelles with Ca2+-sensitive fluorescent proteins
β Scribed by Nicolas Demaurex
- Book ID
- 113525510
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 336 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0143-4160
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## amide gel electrophoresis (1). We have shown (2) by We have established a fluorescence method to detect the overlay technique that calcium ions bind to concalcium-binding proteins by making use of the quinonectin (also called titin), which is a giant myofibrillar line Ca indicator quin2. Authen
Fura-2 and its lipid analogue, FFP-18, were used to measure changes in cytosolic free Ca 2+ concentration within human neutrophils. Whereas fura-2 was employed to monitor cytosolic Ca 2+ increases throughout the cytosol, FFP-18 was used to monitor Ca 2+ changes only near the membrane. This latter pr