Calcium indicator loading of neurons using single-cell electroporation
β Scribed by Thomas Nevian; Fritjof Helmchen
- Book ID
- 106109908
- Publisher
- Springer
- Year
- 2007
- Tongue
- English
- Weight
- 652 KB
- Volume
- 454
- Category
- Article
- ISSN
- 0031-6768
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π SIMILAR VOLUMES
Fluorescent Ca(2+) indicators have been extremely valuable in understanding the role of intracellular Ca(2+). However, the presence of extracellular dye can confound interpretation of data due to indicator accumulation in the Ca(2+)-rich medium, which induces an increase in the fluorescence signal.
Electroporation has been widely accepted as an important tool for the delivery of exogenous molecules into cells. Previous mechanistic studies have been carried out by observing either the average behavior from a large population of cells or the response from a small number of single cells. In this