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Calcium fluxes in rat thyroid FRTL-5 cells. Evidence for Ca2+ entry after stimulation with ATP

✍ Scribed by Törnquist, Kid

Book ID
123367131
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
843 KB
Volume
79
Category
Article
ISSN
0303-7207

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Evidence for receptor-mediated calcium e
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✍ Kid Törnquist 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 824 KB

The aim of the present study was to investigate the relationship between agonist-induced changes in intracellular free CaL+ ([Ca"],) and the refilling of intracellular CaLc stores in Fura 2-loaded thyroid FRTL-5 cells. Stimulating the cells with ATP induced a dose-dependent increase in ([Gal-' 1,).

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Receptor-mediated calcium entry was investigated in Fura 2 loaded FRTL-5 cells. The purinergic agonist ATP activated the release of sequestered calcium and the entry of extracellular calcium. Downregulation of protein kinase C (PKC) substantially enhanced the ATP-evoked calcium entry. Pretreatment o