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Calcium and calmodulin in cellular intoxication with Clostridium difficile toxin B

✍ Scribed by Maria Caspar; Inger Florin; Monica Thelestam

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 522 KB
Volume: 132
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041320124

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✦ Synopsis

In cultured human lung fibroblasts treated with Clostridium difficile toxin B, the development of the cytopathogenic effect was inhibited by the proton ionophore monensin but was not affected by some other ionophores. The calcium channel blockers verapamil and LaCI3 protected the cells against intoxication, as did the calmodulin antagonists trifluoperazine, arnitriptylin, R 24571, and dansylcadaverine. Since these agents could not prevent intoxication when added after the toxin internalization was completed, we suggest that calmodulin and uptake of extracellular calcium are needed for the internalization but not for the cytosolic action of the toxin.

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