Caffeic acid protects human peripheral blood lymphocytes against gamma radiation-induced cellular damage
✍ Scribed by Nagarajan Devipriya; Adluri Ram Sudheer; Venugopal P. Menon
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 584 KB
- Volume
- 22
- Category
- Article
- ISSN
- 1095-6670
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✦ Synopsis
Abstract
In the present study, we investigated in vitro radioprotective potential of caffeic acid (CA), a naturally occurring catecholic acid against gamma radiation‐induced cellular changes. Different concentrations of CA (5.5, 11, 22, 44, 66, and 88 µM) were incubated with lymphocytes for 30 min prior to γ‐irradiation, and micronuclei (MN) scoring and comet assay were performed to fix the effective concentration of CA against γ‐irradiation. Among all concentrations, 66 µM of CA showed the optimum protection by effectively decreasing the MN frequencies and comet attributes. From the above‐mentioned results, 66 µM of CA was selected as the effective concentration and was further used to investigate its radioprotective efficacy. For that purpose, a separate experiment was carried out on the lymphocytes in which lymphocytes were preincubated with CA (66 µM) and were exposed to different doses of radiation (1, 2, 3, and 4 Gy). Genetic damage (MN, dicentric aberration, and comet attributes) and biochemical changes were measured. Gamma‐irradiated lymphocytes showed a dose‐dependent increase in the genetic damage and thiobarbituric acid reactive substances, accompanied by the significant decrease in the antioxidant status, whereas CA pretreatment positively modulated all the radiation‐induced changes through its antioxidant potential. The current study demonstrates that CA is effective in protecting lymphocytes against radiation‐induced toxicity and encourages further in vivo study to evaluate radioprotective efficacy of CA. © 2008 Wiley Periodicals, Inc. J Biochem Mol Toxicol 22:175–186, 2008; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20228