Cadmium Binding Capacity of Cocoa and Isolated Total Dietary Fibre under Physiological pH Conditions
✍ Scribed by Valiente, Cruces; Mollá, Esperanza; Martín-Cabrejas, María M; López-Andréu, Francisco J; Esteban, Rosa M
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 618 KB
- Volume
- 72
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
Cadmium binding capacity of cocoa and isolated total dietary fibre (TDF) was investigated in oitro under simulated physiological pH conditions. The effect of Cd2 + retention on the bioavailability of essential endogenous minerals was also studied. The sample was mixed with buffered Cd2+ solution and, after equilibration, the residue was separated for quantitative measurement of soluble Cd2+ in the supernatant. Cocoa exhibited a high affinity for CdZ+ in solution. The amount of bound Cd2 + rose with increasing metal concentration and pH. TDF was the major fraction responsible for binding of Cd2+ by cocoa. Phytate was probably another important cause of the decrease of CdZ+ solubility. The addition of various concentrations of Cd2+ to cocoa and TDF was shown to affect the solubility of endogenous minerals. When Cd2+ was bound by TDF there was a marked increase in the availability of endogenous Fe, Zn, Ca and Mg, specially at duodenal pH conditions.