Ca2+transport in mitochondrial and microsomal fractions from higher plants

✍ Scribed by Peter Dieter; Dieter Marmé

• Book ID: 104749475
• Publisher: Springer-Verlag
• Year: 1980
• Tongue: English
• Weight: 728 KB
• Volume: 150
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00385606

✦ Synopsis

Mitochondria from etiolated corn possess a much greater Ca 2 + uptake capacity per mg protein than microsomes from the same source. Differences in energy requirements, sensitivity to specific inhibitors, and sedimentation properties enabled us to study both Ca 2 + uptake mechanisms without mutual contamination. The microsomal Ca2 § uptake does not vary much among different plants as compared to the mitochondrial Ca 2 + uptake; this is also true for different organs of the same plant. Mitochondrial Ca 2+ uptake is more dependent on the age of the seedlings than microsomal uptake, because of changes in active Ca 2 + uptake activity rather than of changes in efflux. Intactness and the oxidative and phosphorylative properties of the mitochondria remained unchanged during this time period. Na + and Mg 2+ do not induce Ca 2 + release from mitochondria.