Ca2+ ionophore and phorbol ester stimulate taurine efflux from skate erythrocytes
✍ Scribed by Leite, Margarette V. ;Goldstein, Leon
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 327 KB
- Volume
- 242
- Category
- Article
- ISSN
- 0022-104X
No coin nor oath required. For personal study only.
✦ Synopsis
Hypotonic (approximately 1/31 medium stimulated efflux of taurine from skate (Raja erinacea) RBC approximately twofold at 30 and 60 min incubation. Both Ca2+ ionophore (A23187, lop7 M) and phorbol ester (phorbol 12-myristate 13-acetate, lop7 M) mimicked the effects of hypotonicity on taurine eMux. Calmodulin was probably not involved in the responses to hypotonicity and Ca2 + ionophore, since calmodulin inhibitors did not block either basal taurine release or hypotonic-induced stimulation of taurine release. These results suggest that cell Ca2+ and protein kinase C may both play a role in modulating taurine efflux.