C4 polymorphism in the dog: Molecular heterogeneity of the C4αand C4γsubunit chains

✍ Scribed by Margot Lang; Christoph Nerl; H. Joachim Deeg

• Publisher: Springer-Verlag
• Year: 1984
• Tongue: English
• Weight: 355 KB
• Volume: 20
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00430323

✦ Synopsis

Using an immunoblotting technique and goat antihuman C4, we observed five distinct electrophoretic variants of C4 in a panel of 60 random dogs. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of immunoprecipitated C4 showed that dog C4 is composed of three polypeptide subunit chains (c~,/3, and 7) and that structural variability occurs within the e-and y-chain regions. Two distinct molecular weight forms of both the C4c~-(c~ A and ~B) and C47-(7A and 7B) chain were detected. The variant forms of C4e and C@ were found in association with particular C4 allotypes.