C18 reversed-phase trace enrichment of short- and long-chain (C2C8C20) fatty acids from dilute aqueous solutions and sea water
✍ Scribed by Janusz Pempkowiak
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 702 KB
- Volume
- 258
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
✦ Synopsis
A method is presented for the quantitative determination of aliphatic carboxylic (fatty) acids in dilute aqueous solutions. C,, reversed-phase trace enrichment allows for recoveries ranging from 35 % for acetic acid to 100 y0 for heptanoic and higher acids. The influence of the flow-rate, the matrix and the pH of water on the efficiency of enrichment is established. To enhance detectability, acids are converted into pentafluorobenzyl esters by extractive alkylation. The optimum conditions of derivatization are studied for a wide spectrum of aliphatic acids. The derivatives are analysed by means of capillary column gas chromatography with electron-capture detection. The described method is employed for the determination of fatty acids in sea water. The separating power of a fused-silica capillary column permits the resolution of some 40 peaks which are identified both by correlation of retention times with actual standards and/or by gas chromatography-mass spectrometry.
.