C13 The role of adenosine in the regulation of energy balance in heart and brain

✍ Scribed by David G.L. Van Wylen; Veronica M. Sciolti

• Publisher: Elsevier Science
• Year: 1992
• Tongue: French
• Weight: 152 KB
• Volume: 6
• Category: Article
• ISSN: 0985-0562
• DOI: 10.1016/s0985-0562(05)80388-7

✦ Synopsis

and specific binding sites for the A~ antagonist [3H]DPCPX. The rank order of affinities of various adenosine egonists and antagonists confirmed the identity of the cDNA clone. Binding of agonists was sensitive to the presence of the nonhydrelyzable GTP analog, GppNHp. In edenylyl cyclase essays, adenosine agonists inhibited forskolin-stimulated cAMP production by 50%. Northern and in situ hybddizafion analyses of receptor mRNA in brain tissues revealed two transcripts of 5.6 and 3.1 kb. Both were abundant in cortex, cerebellum, hippocampus and thalamus, with lower levels in olfactory bulb, striatum, mesencephaion and retina. This distribution is in good agreement with receptor autoradiographic atddies of the A~ adenosine receptor. A~ receptor mRNA appears first at mid-embryogenesis (Ef5-17) in lower brain regions (pens, medulla) and in spinal cord. By E2.1 mRNA distribution is abundant and spread throughout the CNS, similar to adult.