C-terminal fragments of parathyroid hormone-related protein, PTHrP-(107-111) and (107-139), and the N-terminal PTHrP-(1-40) fragment stimulate membrane-associated protein kinase C activity in rat spleen lymphocytes
✍ Scribed by James F. Whitfield; Richard J. Isaacs; Balu R. Chakravarthy; Jon P. Durkin; Paul Morley; Witold Neugebauer; Ross E. Williams; Gordon Willick; Raymond H. Rixon
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 587 KB
- Volume
- 158
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Abstract
Membrane‐associated protein kinase C (PKC) activity in lymphocytes freshly isolated from rat spleen was stimulated by the C‐terminal parathyroid hormonerelated protein fragments, PTHrP‐(107–111) and PTHrP‐(107–139), at concentrations from 10^−3^ to 10^4^ pM. By contrast, the same concentrations of PTHrP‐(120–139), Without the 107–111 TRSAW (‐Thr‐Arg‐Ser‐Ala‐Trp‐) sequence of the other C terminal fragments, did not stimulate spleen lymphocyte PKC. Low concentrations of the N‐terminal PTHrP‐(1–40) fragment also stimulated membrane‐associated PKC activity in the spleen lymphocytes. These results suggest that PTHrP might be an important physiological regulator of the immune response. Published 1994 Wiley‐Liss, Inc.