Butane-2,3-Diacetal-Desymmetrized Glycolic Acid—A New Building Block for the Stereoselective Synthesis of Enantiopure α-Hydroxy Acids

✍ Scribed by Elena Díez; Darren J. Dixon; Steven V. Ley

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 100 KB
• Volume: 113
• Category: Article
• ISSN: 0044-8249
• DOI: 10.1002/1521-3757(20010803)113:15<2990::aid-ange2990>3.0.co;2-r

✦ Synopsis

37 8C) and the mixture was filtered in a UltraFree MC 30 000 MWCO centrifugal filtration unit (Millipore) at 3500 g for 7 min at 37 8C. The concentration of free substrate in the filtrate was quantified by ICP-MS and the bound fraction was calculated as % bound ([total]-[free])/[total]. The proton T 1 (longitudinal NMR relaxation time) value of water was measured at 20 MHz at 24 and 37 8C by inversion recovery on a Brucker Minispec; the data were obtained in PBS or with 4.5 % HSA by using 0 ± 40 mm of the Gd 3 complex. The relaxivity (r 1 ) was determined from the slope of the plot of 1/T 1 versus the sample concentration.