Burkitt's lymphoma presenting as ophthalmoplegia in an elderly male
✍ Scribed by Shervl A. Russ; Omar H. Yumen
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 245 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0361-8609
No coin nor oath required. For personal study only.
✦ Synopsis
CAWCAR 13 (35%) 6 (16%) CAWBenin 19(51%) 23 (64%) BeniMBenin 4(11%.) 7 (20%) Others 1(3%) -Blood samples for hematological and DNA analysis were obtained from 73 nonrelated patients with homozygous sickle cell anemia (from the University Hospital of UNICAMP, Campinas, State of Sio Paulo, and from the University Hospital of Federal University of Bahia and of Oncology and Hematology Center "Eric Loffler," Salvador. State of Bahia). The diagnosis was based on clinical and laboratory findings [2].
High molecular weight DNA was isolated from peripheral blood leukocytes with proteinase K or urea using standard procedures [2]. The haplotype determination was carried out by PCR amplification of the region of the polimorfic site followed by digestion with appropriate enzyme. The following restriction sites were analysed: Xmn I of the position -158 of the G-y gene, Hind 111 in IVS2 of *Y. Hind I11 of IVS2 of 'y. Hinc I1 in the $p, Hinc 11 at 3' of JIp and Hinf I at 5' of p. The primers and conditions utilized