Separation of cytoplasts and whole cells
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Leah Ann Lipsich; Joseph J. Lucas; Joseph R. Kates
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Article
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1979
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John Wiley and Sons
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English
β 496 KB
## Abstract Cytoplasts prepared from L929 or Hepaβ2 cells were separated from whole cells using density gradients of renografin. Using this technique, cytoplasts can be isolated from cell lines which cannot be routinely enucleated with an efficiency of 100%. The purified cytoplasts excluded the vit