✦ LIBER ✦

Buoyant density fluctuations during the cell cycle ofBacillus subtilis

✍ Scribed by A. Hart; C. Edwards

Book ID: 104786570
Publisher: Springer
Year: 1987
Tongue: English
Weight: 514 KB
Volume: 147
Category: Article
ISSN: 0302-8933
DOI: 10.1007/bf00492907

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✦ Synopsis

A simple rapid method for preparing synchronous cultures of Bacillus subtilis has been used to investigate changes in density during the cell cycle. Asynchronous cells separated on a stepped Percoll density gradient had a mean cell density of 1.117 g ml-1 +/- 0.004. Samples from a synchronous culture exhibited variation (ca. 1.5%) in mean cell density which was greatest at the onset of cell division. An asynchronous control culture showed little variation in density. These results are discussed in relation to previous work on Escherichia coli.

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