Buoyant densities of natural and synthetic DNA's in CsCl and Cs2SO4 considered as sequence-dependent properties
✍ Scribed by Donald M. Gray; Carla W. Gray; Robert L. Ratliff; David A. Smith
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1974
- Tongue
- English
- Weight
- 469 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
The buoyant densities of natural and synthetic DNA's can be accurately interrelated if second-neighbor influences are taken into account. We derive the following expressions, based partly on the buoyant densities of six synthetic DNA's, for the buoyant densities p (g/cma) of DNA's having random sequences. In CsCI, p = 1.659 + 0.099Hc -O.065Ho2 + 0.080Hca and in CszSOd,
In these equations, HG is the mole fraction of G : C base pairs in the DNA and the buoyant densities are calculated relative to densities for E. cola DNA of 1.703 and 1.426 (g/cm3) in CsCl and Cs~SOd, respectively.