Building-Block Approach for the Straightforward Incorporation of a New FRET (Fluorescence-Resonance-Energy Transfer) System into Synthetic DNA
✍ Scribed by Lilia Clima; Willi Bannwarth
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- German
- Weight
- 365 KB
- Volume
- 91
- Category
- Article
- ISSN
- 0018-019X
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✦ Synopsis
Abstract
The synthesis of the two new phosphoramidites 5 and 8 bearing a carbostyril (=quinolin‐2(1__H__)‐one) chromophore used as donor entity in our recently developed new FRET (fluorescence‐resonance‐energy transfer) system is described (Schemes 1 and 2) The high stability of the chromophore to basic conditions enables the incorporation of the phosphoramidites directly into DNA during solid‐phase synthesis (Schemes 3 and 4). Since this is also possible for the (bathophenanthroline)ruthenium(II) complex used as acceptor (Scheme 4, Steps d and e), the whole labelling procedure to insert the FRET system into synthetic DNA is straightforward and represents a major improvement to our previous strategy.