✦ LIBER ✦

Buffer may be the critical factor in measurement of anti-prothrombin antibody on a γ-ray-irradiated plate by enzyme-linked immunosorbent assay

✍ Scribed by Matsuda, Juzo; Saitoh, Noriko; Tsukamoto, Miyo; Gotoh, Moritaka; Gohchi, Kengo; Kawasugi, Kazuo

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 195 KB
Volume: 53
Category: Article
ISSN: 0361-8609
DOI: 10.1002/(sici)1096-8652(199612)53:4<242::aid-ajh6>3.0.co;2-y

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✦ Synopsis

We investigated the influence of different buffers (Tris-buffer and phosphate buffered saline (PBS)/Tween-20 buffer) on anti-prothrombin antibody (aPT) measurement by enzyme-linked irnmunosorbent assay (ELISA), employing a y-ray-irradiated plate. We found considerable discrepancies in aPT positivity between each buffer, and we suggest that the use of Tris-buffer is not suitable for aPT measurement with a y-ray-irradiated plate to measure aPT.