BSH-CP based 3D solid-state NMR experiments for protein resonance assignment
β Scribed by Shi, Chaowei; Fasshuber, Hannes K.; Chevelkov, Veniamin; Xiang, Shengqi; Habenstein, Birgit; Vasa, Suresh Kumar; Becker, Stefan; Lange, Adam
- Book ID
- 121535849
- Publisher
- Springer Netherlands
- Year
- 2014
- Tongue
- English
- Weight
- 985 KB
- Volume
- 59
- Category
- Article
- ISSN
- 0925-2738
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We present 2D versions of the popular triple resonance HN(CO) CACB, HN(COCA)CACB, HN(CO)CAHA, and HN(COCA) CAHA experiments, commonly used for sequential resonance assignments of proteins. These experiments provide information about correlations between amino proton and nitrogen chemical shifts and
## Abstract In many cases of protein structure determination by NMR a highβquality structure is required. An important contribution to structural precision is stereospecific assignment of magnetically nonequivalent prochiral methylene and methyl groups, eliminating the need for introducing pseudoat