Brown adipose tissue mitochondria are cytochromec— subsaturated

✍ Scribed by Amos S. Gaikwad; T. Ramasarma; C. K. Ramakrishna Kurup

• Book ID: 104672818
• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 509 KB
• Volume: 105
• Category: Article
• ISSN: 0300-8177
• DOI: 10.1007/bf00227751

✦ Synopsis

The oxidative activity of mitochondria freshly isolated from brown adipose tissue of rats was stimulated two-fold on the addition of small concentrations of exogenous cytochrome c to the reaction medium. Loss of membrane-bound cytochrome c did not occur during isolation of mitochondria. Estimation of the high-affinity binding sites on the organelle membrane indicated that less than a third of these sites remained saturated with cytochrome c. The pigment is thus shown to be a functionally limiting electron transport component in brown adipose tissue.