Bromodeoxyuridine treatment of melanoma cells decreases rubidium transport
β Scribed by EVANS, I; KUNG, L; BOSMANN, H; BANERJEE, S
- Book ID
- 122138226
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 239 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0309-1651
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Continuously growing cell cultures, testing positive for tyrosinase activity, were derived from two brain and three lymph-node metastases of five patients with malignant melanoma. These cell cultures were analyzed regarding their proliferation fate with continuous bromodeoxyuridine (BrdUrd) labeling
## Abstract A line of HeLa cells resistant to 5βbromoβ2β²βdeoxyuridine (BUdR) was established by continuous culture in growth medium containing BUdR; during the selection period, BUdR concentrations, initially 15 ΞΌM, were gradually increased to 100 ΞΌM. Cells of a clone (HeLa/B5) established from thi