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Broadly reactive TaqMan® assay for real-time RT-PCR detection of rotavirus in clinical and environmental samples

✍ Scribed by N. Jothikumar; G. Kang; V.R. Hill


Book ID
113767935
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
468 KB
Volume
155
Category
Article
ISSN
0166-0934

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## Abstract A sensitive and specific real‐time RT‐PCR assay to detect rotavirus in stool samples was optimized and validated using a wide range of rotavirus genotypes. The target of the original TaqMan® assay is an 87 bp fragment of the highly conserved non‐structural protein 3 (NSP3) gene. Here we