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Breakdown of the germinal vesicle in bovine oocytes cultivated in vitro

✍ Scribed by Motlík, J. ;Koefoed-Johnsen, H. H. ;Fulka, J.

Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
526 KB
Volume
205
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

Breakdown of the germinal vesicle (GV) was studied in bovine follicular oocytes cultivated in vitro. The intact GV of control oocytes did not correspond to the findings in porcine oocytes (GV I). Instead it was characterized by a nuclear membrane and a few chromocenters in finely granular nucleoplasm (GV II). After two and three hours of cultivation, GV III predominated (50% and 64% respectively). Faintly‐staining chromatin and after a longer interval in the form of filaments also appeared round the chromocenters. After four hours, 35.8% of the oocytes were still at stage GV III but the GV of 46.6% of the oocytes already contained filamentous bivalents (GV IV). After five hours, GV IV (59.7%) was typical, with a less distinct nuclear membrane and condensing bivalents. GV breakdown was completed in 30.4% of the oocytes after five hours of cultivation and in 92.5% after six hours.

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