Brain uptake of α-[14C]methyl-para-tyrosine in the rat

✍ Scribed by P. Cumming; T. K. Venkatachalam; S. Rajagopal; M. Diksic; A. Gjedde

• Book ID: 104600551
• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 615 KB
• Volume: 17
• Category: Article
• ISSN: 0887-4476
• DOI: 10.1002/syn.890170208

✦ Synopsis

The blood-brain permeabilities of L-[3Hltyrosine and the tyrosine hydroxylase (TH) inhibitor a-[14Clmethyl-para-tyrosine ([14ClAMpT) were determined in rat striatum, a brain region rich in TH activity, and in other brain regions containing relatively little TH activity. In striatum, the unidirectional clearance rate (K,) for L-[3Hltyrosine (6.2 ml hg-l mine') was significantly greater than the rates for L-[14ClAMpT (2.8 ml hg-min-l) and D-[l4C1AMPT (0.8 ml hg-l min-l). The apparent volume of distribution (Vf) for L-[14C]AMPT in striatum (72.5 -+ 4.0 ml hg-') did not differ from the V, in other brain regions. The homogeneous distribution of L-[14ClAMPT in rat brain indicates that labeled AMPT is unsuitable for the study of TH in vivo by quantitative autoradiography. o 1994 Wiley-Liss, Inc.