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Brain Glutamate Decarboxylase: Properties of Its Calcium-Dependent Binding to Liposomes and Kinetics of the Bound and the Free Enzyme

✍ Scribed by Manuel Covarrubias; Ricardo Tapia


Book ID
111171229
Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
644 KB
Volume
34
Category
Article
ISSN
0022-3042

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A radiochemical microassay for soluble and membrane-bound glutamate decarboxylase (GAD) is described. Up to 180 samples can be determined per day with a variation coefficient of 2%. The method detects newly synthesized gamma-amino-n-butyric acid in the picomole range and can easily be applied to oth