✦ LIBER ✦

Bradykinin induces superoxide anion release from human endothelial cells

✍ Scribed by James A. Holland; Kirkwood A. Pritchard; Miguel A. Pappolla; Michael S. Wolin; Nancy J. Rogers; Michael B. Stemerman

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 541 KB
Volume: 143
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041430104

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The time‐dependent release of superoxide anion (O) from bradykinin (Bk)‐stimulated human umbilical vein endothelial cells (EC) was measured as the superoxide dismutase‐inhibitable reduction of ferricytochrome C employing a novel application of microspectrophotometry. In the absence of Bk, O release by EC was not detectable. EC exposure to Bk (10^−6^ to 10^−5^ M) resulted in a rapid release of O. The release of O occurred within 5 minutes of exposure. O release was partially inhibited by indomethacin (63 ± 6%), thus suggesting that arachidonic acid metabolism, through cyclooxygenase, contributes to EC O production. EC O release may be an important component in the pathophysiologic actions of Bk on vascular function.

📜 SIMILAR VOLUMES

Superoxide anion release by human endoth

Superoxide anion release by human endothelial cells: Synergism between a phorbol ester and a calcium ionophore

✍ Tsukasa Matsubara; Morris Ziff 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 461 KB

In order to study the signal transduction mechanism of human endothelial cells (EC), the regulation of superoxide anion (02-)release in EC has been investigated using the calcium ionophore A23187 and phorbol myristate acetate (PMA), a potential activator of the Ca2+ activated, phospholipid-dependent

Stimulus interactions in release of supe

Stimulus interactions in release of superoxide anion (O2−) from human neutrophils

✍ James G. Bender; Dennis E. Epps 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 558 KB

Pholasin chemiluminescence detects mostl

Pholasin chemiluminescence detects mostly superoxide anion released from activated human neutrophils

✍ Müller, T. ;Davies, E. V. ;Campbell, A. K. 📂 Article 📅 1989 🏛 John Wiley and Sons ⚖ 763 KB

The bioluminescent oxygen metabolite indicator protein pholasin was characterized with respect to the type and location of reactive oxygen metabolites detected in suspensions of stimulated human neutrophils. Whereas pholasin detected reactive oxygen metabolites from neutrophil suspensions stimulated

Inhibition of Neutrophil Superoxide Prod

Inhibition of Neutrophil Superoxide Production by Adenosine Released from Vascular Endothelial Cells

✍ Gary R. Gunther; Malcolm B. Herring 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 585 KB

Irradiation induces release of Von Wille

Irradiation induces release of Von Willebrand protein from endothelial cells in culture

✍ Lee Ann Sporn; Philip Rubin; Victor Marder; Denisa Wagner 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 255 KB

HIV-1 Nef induces p47phox phosphorylatio

HIV-1 Nef induces p47phox phosphorylation leading to a rapid superoxide anion release from the U937 human monoblastic cell line

✍ Eleonora Olivetta; Cinzia Mallozzi; Vitalba Ruggieri; Donatella Pietraforte; Mau 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 313 KB

## Abstract The Nef protein of the human immunodeficiency virus type 1 (HIV‐1) plays a crucial role in AIDS pathogenesis by modifying host cell signaling pathways. We investigated the effects of Nef on the NADPH oxidase complex, a key enzyme involved in the generation of reactive oxygen species dur