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Bovine serum albumin interacts with bacterial luciferase

✍ Scribed by Makemson, John C. ;Hastings, J. Woodland

Publisher: John Wiley and Sons
Year: 1991
Weight: 396 KB
Volume: 6
Category: Article
ISSN: 0884-3996
DOI: 10.1002/bio.1170060211

No coin nor oath required. For personal study only.

✦ Synopsis

Bovine serum albumin (BSA) affects the amount o f light obtained from bacterial luciferase by competing w i t h luciferase for one o f the luciferase substrates, the aldehyde. A t l o w aldehyde concentrations BSA behaves as an inhibitor, but a t high aldehyde concentrations BSA relieves substrate inhibition. BSA reversibly binds decanal w i t h a Ksi = 3.36 kmol/l. approximately half the affinity o f luciferase for decanal (K, = 1.5 pmol/l). BSA also increased the rate o f intermediate II dark decay. The data suggest that this involves a direct protein-protein (BSA-luciferase) interaction.

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