## Abstract A radioimmunoassay (RIA) for the major internal protein of the bovine leukemia virus (BLV p24) was established using anti‐BLV p24 natural antibodies and purified ^125^I‐labelled BLV p24. The final precipitation of the immune complexes was realized by a preparation of inactivated __Stoph
Bovine leukemia virus specific antibodies among French cattle. I. Comparison of complement fixation and hematological tests
✍ Scribed by D. Levy; L. Deshayes; B. Guillemain; A.-L. Parodi
- Publisher
- John Wiley and Sons
- Year
- 1977
- Tongue
- French
- Weight
- 445 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Abstract
Complement fixation (CF) and hematological studies were performed on 517 cows living in normal conditions in different geographical areas of France. The animals belonged to three different categories: (1) multiple or single case herds, in which lymphosarcomas had been detected in the past five years. (2) Leukemia free herds of high risk regions. (3) Leukemia free and apparently unexposed herds. Positive animals were found with both methods in the first two categories but not in the third. Persistent lymphocytosis and CF antibodies were more frequent in leukemia than in exposed but leukemia free herds. Approximately 2 times more animals were found positive by serologic than by hematologic tests. The mean geometrical titer of CF antibodies was higher in lymphocytotic than in normal animals and highest in lymphosarcomatous cows. Persistent lymphocytosis was first detected in 3‐years‐old animals whereas 22% of younger cows were positive in the CF test.
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## Abstract The control of the spread of BLV infection among cattle requires very sensitive methods of detection. The BLV‐gp5I radioimmunossay presents great advantages over the other serological methods, including the BLV‐p24 radioimmunoassay. This is clear from studies of normal animals from the