𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Bovine fibroblasts in long-term tissue culture: Chromosome studies

✍ Scribed by Folke Lithner; Jan Pontén

Publisher
John Wiley and Sons
Year
1966
Tongue
French
Weight
717 KB
Volume
1
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Three fibroblastic strains of fetal calf lung origin have been grown in vitro in serial culture. Chromosome alterations were found in all three. In early passages the cells remained diploid without chromosome changes, After this period cells started to become hypodiploid until they were all abnormal. The decrease in the counts was caused by a process interpreted as a fusion or association of telocentric elements. In the process telocentric chromosomes were randomly joined two and two at the centromere forming single biarmed chromosomes. No loss of detectable chromatid material accompanied the apparent fusion. No cytological changes were observed in the cultures at this stage.

Around passage 70, additional abnormalities began to appear. Severe chromosome alterations were observed with aneuploidy, widely scattered counts dominated by hypotetraploid numbers, breaks, dicentric forms and various other rearrangements. This change was accompanied by a decreased growth rate and degenerative cytological changes in the cultures analogous to those described in human and hamster fibroblasts approaching the end of their finite life span in vitro.

📜 SIMILAR VOLUMES

Recapitulation of oral mucosal tissues i
Recapitulation of oral mucosal tissues in long-term organotypic culture
✍ Chinnathambi, Sathivel ;Tomanek-Chalkley, Ann ;Ludwig, Nicholas ;King, Elizabeth 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 426 KB

## Abstract To test the influence of fibroblasts on epithelial morphology and expression of keratinocyte proteins and barrier lipids, we bioengineered homotypic and heterotypic oral mucosae and skin using cultured adult human cells. Fibroblasts were allowed to modify collagen type I gels for 2 week

Deposition of collagen fibril bundles by
Deposition of collagen fibril bundles by long-term culture of fibroblasts in a collagen sponge
✍ Berthod, Fran�ois ;Sahuc, Florent ;Hayek, Dany ;Damour, Odile ;Collombel, Christ 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 876 KB

Human fibroblasts cultured for 10 days in a collagen sponge migrated through the pores of the sponge and expressed a moderate mitotic activity, which stabilized after 10 days, and a high collagen and protein synthesis. Between 10 and 27 days, the newly synthesized collagen filled the pores of the sp

Bone marrow chromosomes in acute lymphob
Bone marrow chromosomes in acute lymphoblastic leukaemia: A long-term study
✍ Secker-Walker, Lorna M. ;Swansbury, G. J. ;Lawler, Sylvia D. ;Hardisty, R. M. 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 English ⚖ 858 KB

The bone marrow chromosomes of 25 children with acute lymphoblastic leukaemia (ALL) were examined at diagnosis before treatment, during remission, and in 12 cases, also during relapse. Follow-up was for at least six years. At diagnosis, 17 patients had a major population of chromosomally abnormal ce