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Boundary analysis of sedimentation-velocity experiments with monodisperse and paucidisperse solutes

✍ Scribed by K. E. Van Holde; Wolfgang O. Weischet

Book ID
102761686
Publisher
Wiley (John Wiley & Sons)
Year
1978
Tongue
English
Weight
777 KB
Volume
17
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

A method is presented which allows one to calculate a distribution of sedimentation coefficients from the boundries of sedimentation‐velocity experiments with mono‐ or pauci‐disperse solutes. With two solutes differences in S value as small as 20% can be resolved. In absence of heterogeneity and concentration‐dependent effects, the analysis also provides values for the diffusion coefficient within an accuracy of βˆ’10 to +5%. Tests with both simulated data and ultracentrifugation experiments on short DNA fragments show the value and the limitations of the method.

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