Both open reading frames of the linear plasmid pMC3-2 from the ascomyceteMorchella conicaare transcribed in vivo

✍ Scribed by M. Rohe; F. Meinhardt

• Book ID: 104722976
• Publisher: Springer-Verlag
• Year: 1992
• Tongue: English
• Weight: 339 KB
• Volume: 22
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00326418

✦ Synopsis

Mitochondrial RNA was isolated from the morel strain Morchella conica 3 harbouring the linear plasmid pMC3-2 and subjected to gel electrophoresis followed by a Northern analysis using cloned fragments of the plasmid pMC3-2 as probes. Hybridization was obtained only with central parts of pMC3-2 and specific bands of mtRNA. The hybridization bands (2.8 kb and 1.0 kb) correspond in size to the length of the two ORFs of pMC3-2 which were deduced from nucleotide-sequence data. Thus, both ORFs, one encoding a DNA polymerase and the other a yet unknown protein, are transcribed in the mitochondria of the plasmid-bearing Morchella conica strain.