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Bone tissue formation by mouse bone marrow cell suspension in organ cultures

โœ Scribed by E. A. Luria; S. A. Kuznetsov; E. N. Genkina; A. Ya. Fridenshtein

Publisher
Springer US
Year
1988
Tongue
English
Weight
888 KB
Volume
105
Category
Article
ISSN
0007-4888

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Fresh marrow cells were obtained from the femora Fischer rats and cultured in a medium containing 15% fetal calf serum (FCS) to leach confluent. After trypsinization, cells were subcultured at a cell density of 100 X 103/35 mm well in the presence of FCS, 10 mM P-glycerophosphate, 82 p g / mL ascorb