Bone morphogenetic protein 2 stimulates articular cartilage proteoglycan synthesis in vivo but does not counteract interleukin-1α effects on proteoglycan synthesis and content
✍ Scribed by Harrie L. Glansbeek; Henk M. van Beuningen; Elly L. Vitters; Peter M. van der Kraan; Wim B. van den Berg; Elisabeth A. Morris
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 1017 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0004-3591
No coin nor oath required. For personal study only.
✦ Synopsis
Objective. To study the effect of bone morphogenetic protein 2 (BMP-2) on articular cartilage proteoglycan (PG) synthesis in vivo and to investigate whether BMP-2 is able to counteract the effects of interleukin-1 (IL-1) on articular cartilage PG synthesis and content.
Methods. BMP-2 alone or in combination with IL-la was injected into murine knee joints. PG synthesis was measured by 3sS-sulfate incorporation using an ex vivo method or autoradiography. Cartilage PG content was analyzed by measuring Safranin 0 staining intensity on histologic sections.
Results. BMP-2 appeared to be a potent stimulator of articular cartilage PG synthesis in vivo. However, BMP-2 was not able to counteract the deleterious effects of IL-la on articular cartilage PG synthesis and content. In addition, intraarticular injections of BMP-2 induced chondrophytes.
Conclusion.
Although BMP-2 is a very potent stimulator of cartilage PG synthesis in vivo, the therapeutic applications of BMP-2 are limited due to the inability of BMP-2 to counteract the effects of IL-1 and the induction of chondrophytes.
Rheumatoid arthritis (RA) is a disease characterized by chronic inflammation of the joints. The dis-