Bone marrow-derived stromal cell line expressing osteoblastic phenotype in vitro and osteogenic capacity in vivo

✍ Scribed by D. Benayahu; Y. Kletter; D. Zipori; S. Wientroub

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 986 KB
• Volume: 140
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041400102

✦ Synopsis

Marrow stroma has been shown to have osteogenic potential. Here we report the characterization of a unique stromal cell line derived from mouse bone marrow (MBA-15), which expresses osteoblastic phenotype in vitro and forms bone in vivo. More than 70% of cells in culture were histochemically positive for alkaline phosphatase. The enzyme levels were enhanced threefold when cultures were treated with dexamethasone. Gel electrophoresis of [3HJ-proline-labeled cultures showed that MBA-15 cells produced only type I collagen. These cells were responsive to PTH, as indicated by a 50-fold increase in intracellular CAMP. Prostaglandin EZ, but not calcitonin, stimulated CAMP up to 70-fold. When cultures were grown to confluence and fed daily with ascorbic acid and P-glycerophosphate, the cells formed a Von Kossa positive, thick extracellular matrix, shown to contain hydroxyapatite crystals. MBA-15 cells produced mineralized bone when implanted in diffusion chambers. These results indicate that the MBA-15 cell line possesses osteoblastic features in vitro and osteogenic capacity in vivo.