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Bone Marrow and Stem Cell Transplantation

✍ Scribed by Beksaç, Meral


Publisher
Humana Press
Year
2014
Tongue
English
Leaves
320
Series
Methods in Molecular Biology Methods and Protocols 1109
Edition
2nd ed.
Category
Library

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✦ Synopsis


Reporter Gene Technologies for Imaging Cell Fates in Hematopoiesis -- Flow Cytometry for Hematopoietic Cells -- Isolation and Characterization of Mesenchymal Stem Cells -- Methods for Functional Analysis of Stem Cells -- An Overview of HLA Typing for Heamatopoietic Stem Cell Transplantation -- HLA Typing with Sequence-Specific Oligonucleotide Primed PCR (PCR-SSO) and Use of the Luminexβ„’ Technology -- Sequence Based Typing of HLA: An Improved Group Specific Full Length Gene Sequencing Approach -- Molecular Typing Methods for Minor Histocompatibility Antigens -- Natural Killer Cells and Killer-cell Immunoglobulin-like Receptor Polymorphisms: Their Role in Hematopoietic Stem Cell Transplantation -- Methods of Detection of Immune Reconstitution and T Regulatory Cells by Flow Cytometry -- Molecular Methods Used for Detection of Minimal Residual Disease Following Hematopoietic Stem Cell Transplantation in Myeloid Disorders -- Molecular Methods for Detection of Minimal Residual Disease Following Transplantation in Lymphoid and Plasma Cell Disorders -- Molecular Methods for Detection of Invasive Fungal Infections and Mycobacteria and Their Clinical Significance in Hematopoietic Stem Cell Transplantation -- Post-transplant Monitoring of Chimerism by Lineage-specific Analysis -- Urinary Proteomics Employing Capillary Electrophoresis Coupled to Mass Spectrometry in The Monitoring Of Patients After Stem Cell Transplantation.;The second edition of Bone Marrow and Stem Cell Transplantation expands upon the previous edition with current, detailed methods on HLA, minor-HLA and Killer Immunoglobulin Like Receptor typing. With new chapters on immunophenotyping and functional characterization of stem cells are included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bone Marrow and Stem Cell Transplantation, Second Edition serves as a guide in the application of molecular methods for routine or investigational purposes.

✦ Table of Contents


Reporter Gene Technologies for Imaging Cell Fates in Hematopoiesis --
Flow Cytometry for Hematopoietic Cells --
Isolation and Characterization of Mesenchymal Stem Cells --
Methods for Functional Analysis of Stem Cells --
An Overview of HLA Typing for Heamatopoietic Stem Cell Transplantation --
HLA Typing with Sequence-Specific Oligonucleotide Primed PCR (PCR-SSO) and Use of the Luminexβ„’ Technology --
Sequence Based Typing of HLA: An Improved Group Specific Full Length Gene Sequencing Approach --
Molecular Typing Methods for Minor Histocompatibility Antigens --
Natural Killer Cells and Killer-cell Immunoglobulin-like Receptor Polymorphisms: Their Role in Hematopoietic Stem Cell Transplantation --
Methods of Detection of Immune Reconstitution and T Regulatory Cells by Flow Cytometry --
Molecular Methods Used for Detection of Minimal Residual Disease Following Hematopoietic Stem Cell Transplantation in Myeloid Disorders --
Molecular Methods for Detection of Minimal Residual Disease Following Transplantation in Lymphoid and Plasma Cell Disorders --
Molecular Methods for Detection of Invasive Fungal Infections and Mycobacteria and Their Clinical Significance in Hematopoietic Stem Cell Transplantation --
Post-transplant Monitoring of Chimerism by Lineage-specific Analysis --
Urinary Proteomics Employing Capillary Electrophoresis Coupled to Mass Spectrometry in The Monitoring Of Patients After Stem Cell Transplantation.

✦ Subjects


Analytical, Diagnostic and Therapeutic Techniques and Equipment;Anatomy;Antigens;Antigens, Surface;Biological Factors;Bone Marrow Cells;Bone marrow--Transplantation;Bone Marrow Transplantation;Cell Transplantation;Cells;Chemicals and Drugs;Diagnosis;Hematopoietic Stem Cell Transplantation;Hematopoietic Stem Cells;Hematopoietic System;Hemic and Immune Systems;Histocompatibility Antigens;Histocompatibility Testing;HLA Antigens;Immunologic Techniques;Immunologic Tests;Investigative Techniques;Isoan


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