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Bone formation by culture-expanded bone marrow cells exposed to growth factors in vitro and in vivo

โœ Scribed by M. Higashinakagawa; I. Asahina; S. Enomoto

Book ID
117611689
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
115 KB
Volume
26
Category
Article
ISSN
0901-5027

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Fresh marrow cells were obtained from the femora Fischer rats and cultured in a medium containing 15% fetal calf serum (FCS) to leach confluent. After trypsinization, cells were subcultured at a cell density of 100 X 103/35 mm well in the presence of FCS, 10 mM P-glycerophosphate, 82 p g / mL ascorb