Bone biopsy derived marrow stromal elements rescue chronic lymphocytic leukemia B-cells from spontaneous and drug induced cell death and facilitates an “angiogenic switch”
✍ Scribed by Neil E. Kay; Tait D. Shanafelt; Ann K. Strege; Yean K. Lee; Nancy D. Bone; Azra Raza
- Publisher
- Elsevier Science
- Year
- 2007
- Tongue
- English
- Weight
- 952 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0145-2126
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✦ Synopsis
A novel bone biopsy technique was used to generate a robust stromal cell system to study how stroma modulates CLL B-cell apoptosis and how the leukemic cell-stromal interaction influences secretion of vascular factors. Marrow stromal elements (MSE) rescued CLL B-cells from both spontaneous and drug induced apoptosis, partly due to soluble factors. When CLL B-cells were added to the MSE cultures, a dramatic increase in the secretion of basic fibroblast growth factor and decrease in the secretion of thrombospondin was observed. These results indicate the interaction between CLL B-cells and marrow stromal elements regulates angiogenic switching and may be linked to disease progression.