✦ LIBER ✦

Bombesin may stimulate proliferation of human pancreatic cancer cells through an autocrine pathway

✍ Scribed by Qiming J. Wang; Joseph A. Knezetic; Andrew V. Schally; Parviz M. Pour; Thomas E. Adrian

Publisher: John Wiley and Sons
Year: 1996
Tongue: French
Weight: 883 KB
Volume: 68
Category: Article
ISSN: 0020-7136
DOI: 10.1002/(sici)1097-0215(19961115)68:4<528::aid-ijc20>3.0.co;2-#

No coin nor oath required. For personal study only.

✦ Synopsis

Bombesin is trophic to normal pancreas and acinar cell adenocarcinoma, but its effects on d u d cell tumors are undetermined. The autocrine growth effects of bombesin on well-differentiated (HPAF, CDI I) and poorly differentiated (CD18, PANC-I) human d u d pancreatic cancer cell lines were investigated. Receptor binding of labeled bombesin was measured in a whole-cell microplate assay. Bombesin production was measured by radioirnmunoassay. Proliferative responses were quantified using the M l T assay. Messenger R N A for bombesin and its receptor were identified by primer extension analysis. A single class of high-affinity binding sites was detected on HPAF and CD18 cells. Similar affinities and high receptor densities were found on the 2 cell lines. Bombesin was secreted by all 4 cell lines during 24-hr culture in serum-free media, and its recovery was enhanced in the presence of protease inhibitors. Primer extension analysis demonstrated ~

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