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BMP-4 affects the differentiation of metanephric mesenchyme and reveals an early anterior-posterior axis of the embryonic kidney

✍ Scribed by Anne Raatikainen-Ahokas; Marjo Hytönen; Auri Tenhunen; Kirsi Sainio; Hannu Sariola


Book ID
102651826
Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
727 KB
Volume
217
Category
Article
ISSN
1058-8388

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✦ Synopsis


Bone morphogenetic protein-4 (BMP-4), a member of the transforming growth factor-␤ (TGF-␤) family, regulates several developmental processes during animal development. We have now studied the effects of BMP-4 in the metanephric kidney differentiation by using organ culture technique. Human recombinant BMP-4 diminishes the number of ureteric branches and changes the branching pattern. Our data suggest that BMP-4 affects the ureteric branching indirectly via interfering with the differentiation of the nephrogenic mesenchyme. The clear positional preference of the defects to posterior mesenchyme might reflect an early anterior-posterior patterning of the metanephric mesenchyme. The smooth muscle ␣-actin expressing cell population around the ureteric stalk, highly expressing Bmp-4 mRNA, is also expanded in kidneys treated with BMP-4. Thus, BMP-4 may be a physiological regulator of the development of the periureteric smooth muscle layer and ureteric elongation.