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Blue sepharose: a reusable affinity chromatography medium for purification of alcohol dehydrogenase

โœ Scribed by Geraldin E. Lamkin; E.E. King


Book ID
119406172
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
238 KB
Volume
72
Category
Article
ISSN
0006-291X

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Rapid large-scale purification of pig he
โœ I. Lascu; M. Duc; A. Cristea ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 436 KB

A rapid procedure for the large-scale purification of pig heart nucleoside diphosphate kinase is described. The purification procedure involves extraction of the enzyme, absorption on Cibacron Blue 3G-A Sepharose, elution with ATP, ammonium sulfate precipitation, heat treatment, and rechromatography