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Blue-native PAGE in plants: a tool in analysis of protein-protein interactions

✍ Scribed by Holger Eubel; Hans-Peter Braun; A Harvey Millar


Book ID
119910234
Publisher
BioMed Central
Year
2005
Tongue
English
Weight
873 KB
Volume
1
Category
Article
ISSN
1746-4811

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Differential gel electrophoresis (DIGE) is based on pre-labeling of different protein fractions and their subsequent co-electrophoresis in a single gel. Cyanine based "CyDye DIGE Fluor minimal dyes" are used for the labeling reaction and 2D IEF/SDS PAGE is the preferential electrophoresis system for