Blotting Membrane Micropreparation in Capillary Electrophoresis to Evaluate Enzyme Purity and Activity

✍ Scribed by T. Konse; T. Takahashi; H. Nagashima; T. Iwaoka

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 217 KB
• Volume: 214
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1993.1474

✦ Synopsis

A micropreparation system for proteins and peptides analyzed by capillary electrophoresis that uses a blotting membrane and an improved micro-test-plate was developed. The system is useful for enzyme diagnosis and purification. In the system, enzyme is collected in a concentrated spot on these membranes, and the activities are measured enzymatically to evaluate the resolution of the system. 1993 Academic Press, Inc.