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Blood platelet adhesion to printed von Willebrand factor

✍ Scribed by Polina Davydovskaya; Marek Janko; Florian Gaertner; Zerkah Ahmad; Özlem Simsek; Steffen Maßberg; Robert W. Stark

Book ID
102875079
Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
991 KB
Volume
100A
Category
Article
ISSN
1549-3296

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✦ Synopsis

Abstract

Von Willebrand factor (vWF), a glycoprotein in blood, mediates the adhesion of blood platelets and thus plays a crucial role in hemostasis and thrombosis. Functional coating of surfaces with vWF allows the investigation of in vitro adhesion of blood platelet. We used soft lithography to create a functional patterned substrate. vWF was printed on plasma‐treated glass and mica surfaces, producing elongated network‐like fibril structures. A minimum layer thickness of 3 nm was observed, corresponding to the height of a monolayer of vWF. The stability of the patterns was verified in a laminar fluid flow, and the bioactivity of the structures was tested with platelet adhesion experiments. Platelets adhered to and spread on printed vWF. These results indicate that printed vWF substrates are stable and functional in typical perfusion experiments, and thus provide a useful tool for studying thrombus formation in vitro. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.

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