Blood permeability of a novel ceramic scaffold for bone morphogenetic protein-2

✍ Scribed by M. Murata; T. Akazawa; J. Tazaki; K. Ito; T. Sasaki; M. Yamamoto; Y. Tabata; M. Arisue

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 414 KB
• Volume: 81B
• Category: Article
• ISSN: 1552-4973
• DOI: 10.1002/jbm.b.30686

✦ Synopsis

Abstract

A functionally graded apatite (fg‐HAp) with body fluid permeability was developed from bovine bone. The tissue reaction of fg‐HAp and its efficacy as a scaffold for recombinant human bone morphogenetic protein‐2 (BMP‐2) were evaluated histomorphometrically, and a component of permeable fluid into the fg‐HAp was analyzed by immunoblotting assay. The fg‐HAp block (27 mm^3^) combined with and without BMP‐2 (5 μg) was implanted subcutaneously in 4‐week‐old Wistar rats. Histological examination showed that the surface and bulk degradations of the fg‐HAp proceeded extensively and giant cells appeared on the fg‐HAp at 2 weeks. Body fluid permeation was found inside the fg‐HAp, and the fluid component was immunopositive for albumin. In addition, albumin was detected as a main component among proteins collected from the in vivo implanted fg‐HAp. The bioabsorption of the fg‐HAp was accelerated as BMP‐2‐induced bone matured. Histomorphometrical analysis at 4 weeks in the BMP‐2/fg‐HAp implant showed 59.0% in the total volume of bone and marrow. These results indicate that fg‐HAp is an innovative, bioabsorbable bioceramic with fluid permeability characteristic, and may become a biointegrated scaffold for bone engineering. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006