Bleaching of chlorophylls in alcohol extracts with benzoyl peroxide for liquid scintillation counting of 14C-labeled compounds
✍ Scribed by Paul N.P. Chow
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 334 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Benzoyl peroxide in toluene was used to bleach chlorophylls in alcohol extracts from plants fed with r4C0, for improved direct measurement by liquid scintillation counting. The 0.5% benzoyl peroxide was better than the saturated solution due to less color quenching. Increased counts occurred with all five common scintillation systems when the leaf extracts were bleached with the 0.5% benzoyl peroxide. However, the relatively colorless root extracts of Y-labeled photosynthates in alcohol did not require the addition of benzoyl peroxide for decolorization. The addition of 0.5 ml of benzoyl peroxide solution (0.5%) to 0.5 ml of a solution of 10 Y-labeled compounds did not induce the degradation of these compounds. In contrast, sodium hypochlorite at an equal concentration caused considerable losses of radioactivity, with an overall average of 30% reduction of the 10 "'C-labeled compounds examined.