## Abstract The in vivo incorporation of ^3^H‐uridine into RNA was studied in delayed implanting and activated blastocysts obtained from 33 western spotted skunks. ^3^H‐uridine was incorporated into RNA by all blastocysts; however, significantly more label was incorporated as blastocyst diameter in
Blastocyst protein synthesis during obligate delay of implantation and embryo activation in the western spotted skunk
✍ Scribed by Rourke, A. W. ;Mead, Rodney A.
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 504 KB
- Volume
- 221
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
The in utero protein synthetic activity of embryos of the western spotted skunk has been examined by one‐dimensional gel electrophoresis and fluorography. The results indicate that delayed‐implanting embryos synthesize a large variety of proteins with molecular weights from < 15,000 to > 200,000. Delayed‐implanting blastocysts synthesize several very large proteinaceous molecules which do not appear to be produced by activated, preimplantation embryos. Activated embryos show increased synthetic activity, but there are many qualitative similarities in the molecular weight profiles characteristic of delay and activated blastocysts.
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## Abstract The pars tuberalis of the western spotted skunk is the only region of the brain known to bind 2‐[^125^I]iodomelatonin, thus suggesting that this region of the pituitary might be essential in mediating the effects of melatonin in this species. Female skunks were killed during short‐and l